PCR-based Diagnosis of Mucormycosis Targeting Mucorales-specific Genes

PCR-based Diagnosis of Mucormycosis Targeting Mucorales-specific Genes

Molecular methods to improve diagnosis and identification of mucormycosis.

Mucormycosis is difficult to diagnose. Samples from suspected cases often fail to grow Mucorales in microbiologic cultures. We identified all hematologic malignancy and stem cell transplant patients diagnosed with proven mucormycosis between 2001 and 2009 at Brigham and Women's Hospital/Dana-Farber Cancer Institute. Seminested PCR targeting Mucorales 18S ribosomal DNA and sequencing were perfor...

Breath-Based Diagnosis of Invasive Mucormycosis (IM)

1776. Breath-Based Diagnosis of Invasive Mucormycosis (IM) Seena Koshy, PhD; Nour Ismail, MD; Carmen Leon Astudillo, MD; Christina Mallarino Haeger, MD; Obadah Aloum, MBBS; Mahesh Thalavitiya Acharige, PhD; Dimitrios Farmakiotis, MD; Lindsey R. Baden, MD; Francisco M. Marty, MD, FIDSA; Dimitrios P. Kontoyiannis, MD, ScD, PhD (Hon), FACP, FIDSA, FECMM, FAAM; Laxura Fredenburgh, MD and Sophia Koo...

Is It Time to Include CT “Reverse Halo Sign” and qPCR Targeting Mucorales in Serum to EORTC-MSG Criteria for the Diagnosis of Pulmonary Mucormycosis in Leukemia Patients?

In 23 leukemia patients with proven (n = 17) or possible (n = 6) pulmonary mucormycosis (PM), the presence of reversed halo sign on computed tomography was strongly associated with the positivity of quantitative polymerase chain reaction assays targeting Mucorales in the serum, confirming the value of these two tools for the diagnosis of PM in this setting.

Quantitative polymerase chain reaction detection of circulating DNA in serum for early diagnosis of mucormycosis in immunocompromised patients.

BACKGROUND The aim of our study was to assess the detection of circulating DNA from the most common species of Mucorales for early diagnosis of mucormycosis in at-risk patients. METHODS We retrospectively evaluated a combination of 3 quantitative polymerase chain reaction (qPCR) assays using hydrolysis probes targeting Mucor/Rhizopus, Lichtheimia (formerly Absidia), and Rhizomucor for circula...